| Summary: | 碩士 === 國立臺灣大學 === 應用力學研究所 === 104 === In current development for cardiovascular drug discovery, the main parameters are the cardiac systolic and diastolic profiles, beating frequency, and contractile profile. Among these parameters, cardiac systolic and diastolic profiles are the most common, and the monitoring systems are usually based on flexible substrates fabricated by MEMS based microfluidic system is widespread. However, the systems for monitoring cardiac systolic and diastolic profiles are mostly based on an optical systems, and the force profiles is estimated from calculation of the deformation of cells or flexible substrates. Hence, the detection is not direct and could not directly infer relationship between cardiac contraction and drug.
To achieve a fully automatic, real-time and direct massive cardiac drug monitoring system, a platform for real-time monitoring cardiac contractile profile was developed in this study. A piezoelectric material, [poly[(vinylidenefluoride-co-trifluoroethylene]; P(VDF-TrFE:75/25)], was chosen to be the core of the transducer. It is composed of multiple nanofibers to create piezoelectric nanofiber bundles. In order to rapidly develop nanofiber bundles, the electrospinning method was applied. The parallelly oriented piezoelectric nanofiber bundles could be massively fabricated. The overall platforms could be fabricated in one day. A biomimetic substrate coating for facilitating cardiomyocyte adhesion and maturation was also developed. Furthermore, an interface system for monitoring contraction of cardiac micro-tissue was developed and could directly and automatically convert the mechanical force of cardiomyocyte to electrical signals. Also, we verified that this platform can detect cardiac contractile profile by administrating Isoproterenol and Verapamil compounds.
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