Target Cell Electroporation in Microfluidic Device

• Main Authors: Sheng-Kai Huang, 黃聖凱
• Other Authors: 盧彥文
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/34314854527398730592

碩士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 104 === One of the key techniques in gene delivery and cell therapy is to break through the limit of the cell membrane and to send gene or drug into the cell. Electroporation is one of such methods. However, it conventionally suffers the drawbacks in high voltage, large sample amount and high heat generation. The gene/drug delivery results can be only estimated for the average gene expression of the group of the cells, instead of a single cell. To realize a particular gene expression onto a single cell, our thesis thus is devoted to perform electroporation on a single cell while to improve the limitation of the traditional electroporation techniques. A microfluidic device for single cell electroporation was presented. The HEK 293T cells were tested. The cells were first confined at the L-shape trapping structure with cross-flow cell filters enhanced by hydrodynamic focusing forces. Once the cells were trapped, the different voltage were applied for electroporation. Fluorescence dye and pEGFP-c1 plasmid were successfully delivered into the cells to validate the devices.