HMYB1 interacts with HDA6 and is involved in flowering in Arabidopsis

• Main Authors: Yu-Hao Ke, 柯昱豪
• Other Authors: 吳克強
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/38501509319270870251

碩士 === 國立臺灣大學 === 植物科學研究所 === 104 === Histone deacetylases mediate histone deacetylation and act in concert with histone acetyltransferases to regulate dynamic and reversible histone acetylation, which modifies chromatin structure and affects gene transcription. By using yeast two hybrid library screening and LC-mass/mass analysis, many proteins that interact with HISTONE DEACETYLASE 6 (HDA6) were identified. The interaction of HDA6 with these identified proteins was further confirmed by bimolecular fluorescence complementation assays. HMYB1 (At1G25550) and TRFL5 (At1G15720), two MYB transcription factors interacting with HDA6, were selected to further study their function. Our results show that their MYB domains are required for the interaction with HDA6 and HMYB1 is transcriptional repressor by dual-luciferase reporter assay. HMYB1 is highly expressed in roots and rosette leaves, but TRFL5 is highly expressed in seeds Furthermore, HMYB1 overexpression plants (HMYB1-OE) show delayed flowering, and FLC is upregulated but FT is downregulated in HMYB1-OE plants. We also generated HMYB1 knockdown transgenic plants (Ami-HMYB1) by using artificial microRNA. Ami-HMYB1 plants exhibit early flowering. Taken together, our results indicate that HMYB1 is a transcriptional repressor interacting with HDA6 and is involved in flowering in Arabidopsis.