| Summary: | 碩士 === 國立臺灣大學 === 物理研究所 === 104 === Many results in cellular traction force microscopy (TFM) based on the flat matrix deformation determined by fluorescent marker displacement has been produced. Cells, however, are naturally live in three-dimensional environment, so we tried to make a substrate contain symmetrical spherical hole with tunable curvature to measure traction force. We use confocal microscope to image live cells. In order to carry out live cell imaging, the crucial factor is the heated environment for cell to live. The temperature fluctuation produced in the optical system made focus plane drift caused by the thermal expansion of lens or materials. In order to determine marker displacement in sub-micrometer scale, I did the temperature control experiment. I built a temperature-controlled microscope system by using our own microscope, and tested whether thermal drift effect was eliminated. Finally, I tried different heating methods to estimate the thermal drift quantity from the temperature increasing of objectives.
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