Implementing Soil Washing for Soil Remediation and Using Rhodococcus erythropolis NTU-1 for the Treatment of the Residual Solution

• Main Authors: Yao-Chang Yang, 楊曜彰
• Other Authors: 劉懷勝
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/4cndjg

碩士 === 國立臺灣大學 === 化學工程學研究所 === 104 === For bioremediation, we focused on how to apply soil washing method with MSM (mineral salt medium) to wash n-hexadecane (n-C16) on soil. Furthermore, we apply Rhodococcus erythropolis NTU-1 (NTU-1) biofloccules and biodegradation for n-hexadecane removal in MSM. Firstly, results showed that nearly 800 ppmv n-C16 can be washed by 100 ml MSM from 0.5 g contaminated soil of which the particle size is 20/50 mesh and the added volume of pollutant is 4 ml n-C16/g soil. By analyzing with GC-FID, soil will contain 3.2 ml n-C16/g soil after added n-C16 as pollutant. Secondly, to avoid hydrosoluble compounds in soil being desolved in MSM during soil washing, which may constrain the biodegradation ability of NTU-1, we have to pre-wash soil. Being pre-washed by both tap water and DI water, soil can be used for subsequent experiments. After incubated in MSM used to wash contaminated soil for 72 hours, NTU-1 will remove approximately 95% of total n-C16 in MSM by both biodegradation and biofloccules. Last but not least, the washing effectiveness of MSM can be promoted by increasing the temperature of MSM. Results showed almost 20% of total n-C16 can be removed from 0.5 g soil whose particle size is 20/50 mesh and the added volume of pollutant is 4 ml n-C16/g soil during soil washing at 100℃. After 72 hours NTU-1 incubation, almost 95% of total n-C16 in MSM will be removed.