| Summary: | 碩士 === 國立臺灣海洋大學 === 生命科學暨生物科技學系 === 104 === Although the medical technology advances, cancer still sustained high level causes of death in people, we can see the current understanding of cancer is still quite limited. Due to cell abnormal proliferation as well as transfer capability by all possible via pipeline (eg: the lymphatic system and blood vessels) of adjacent organs or tissue, tumor cells can cause lesions and other body parts of complications.
Previous research shows that microRNA is a small non-coding RNA fragment playing an important role to regulate the gene expression. The expression of microRNA-7a is tightly regulated and restricted predominantly to the brain, spleen and pancreas. The reduction of microRNA-7a have been linked to the development of cancer and metastasis. In the past literature showed that as a tumor suppressor, microRNA-7a can inhibit oncogene such as EGFR、YY1、RAF1、BCL-2 (Kalinowski et al. (2014)). By downregulating numbers of oncogene, microRNA-7a has been successfully halted tumor cell proliferation no matter in vitro or in vivo. Since only very few studies related loss-of-zebrafish as the study model organism and microRNA-sponge approach to address the functional role of microRNA-7a in the mechanism of tumorigensis (Pollock et al. (2014)). We found that downregulated microRNA-7a level caused oncogne(EGFR,YY1,RAF1…) level has been upregulated. Put together, our microRNA-sponge transgenic fish model provides a new way to study the new treatment of cancer in the future.
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