| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 104 === Phenolphthalein has been used as a drug for the treatment of constipation. Animal experiments show that phenolphthalein is carcinogenic and genotoxic. It can cause genetic damage of p53 tumor suppressor. Using phenolphthalein as a drug or adding it to the health functional food may be harmful to the human health. At present, many countries, including Taiwan, the United States, Japan, Canada, and some European countries prohibit the sale of laxative or health functional food which contains phenolphthalein.
Different extraction conditions were tried to extract phenolphthalein from laxatives and health functional food. An optimal analytical procedure was also established for quantification of phenolphthalein by the use of high performance liquid chromatograph (HPLC) along with photodiode array (PDA) detector. Commercial laxatives and health functional food were analyzed with the procedure established in this research to see if phenolphthalein was added.
An InertSustain® C18 column was used and the detector wavelength was set at 228 nm. A mobile phase of acetonitrile/water (45:55, v/v) isocratically eluted the analyte. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.0192 and 0.0638 ppm, respectively.
Samples were extracted by ultrasonic assisted extraction (UAE) method and microwave assisted extraction (MAE) method, respectively. The optimum extraction condition for phenolphthalein tablet was to perform ultrasonic assisted extraction for 5 minutes. The recovery of phenolphthalein was 86.7 (± 0.1) ~ 95.8 (± 0.1)%. The optimum extraction condition for health functional food powder A was to perform microwave assisted extraction with the power of 200 W for 2 minutes. The recovery of phenolphthalein was 80.43 (± 0.04) ~ 96.13 (± 0.03)%. The optimum extraction condition for health functional food powder B was to perform ultrasonic assisted extraction for 45 minutes. The recovery of phenolphthalein is 84.0 (± 0.2) ~ 94.0 (± 0.2)%. The results showed that illegal addition of phenolphthalein still exists in the domestic market. Determination of phenolphthalein content of the health functional food should be strengthened in order to ensure the food safety.
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