| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 104 === Ganoderma lucidum has been used for over 2000 years in traditional Chinese medicine. The culture medium and condition for the solid-state cultivation of G. lucidum mycelia had been established previously. One of the aims of this study was to investigate the effect of ventilation on the production of the bioactive substances of glucan, triterpene and γ-aminobutyric acid (GABA) by G. lucidum using a novel solid-state fermenter. After 28 days of cultivation, the protein content of G. lucidum fermented product is elevated form 11.58 ± 0.01% to 21.37 ± 0.01%; the lipid content is elevated from 2.97 ± 0% to 4.38 ± 0%. The G. lucidium fermented product was extracted by 10% ethanol (extract A), 70oC warm water (extract B) and hot water following alcohol precipitation (extract C). All extracts contained the three bioactive substances of glucan, triterpene and GABA, with the extract A and extract B being the highest GABA content and the extract C the highest glucan content. The protective effects of the 3 extracts of G. lucidum fermented products on H2O2 (100 μM)–damaged neuroblastoma SH-SY5Y cells were further evaluated. Pretreatment of cells with the extracts of A and B at 0.26 μg/mL, followed by H2O2 damage, significantly increased cell viability by 37.5% and, 48.9%, respectively; while the extract C did not significantly increase cell viability. In addition, after being damaged by H2O2 and followed by the treatment with the extract A and B, the cell viability was significantly increased by 38.6% and 47.0%, respectively; while the extract C did not significantly increase cell viability. A significant increase in superoxide dismutase (SOD) activity was observed in the extract B treated group and GABA standard. These results suggested that GABA component in G. lucidum fermented product is the major contributor for protection of SH-SY5Y cells from oxidative damage. In conclusion, this study revealed that the G. lucidum fermented GABA-rich product has potential to prevent neuronal oxidative damage as well as to facilitate neuronal recovery.
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