| Summary: | 碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 104 === The first experiment was conducted to determine the effect of dietary Peruvian fish meal (PFM) protein replaced by Taiwanese fish meal (TFM) protein on the growth performance of orange-spotted grouper Epinephelus coioides. Six isonitrogenous (45.8% crude protein) and isolipidic (11.4% crude lipid) diets were formulated to contain 0, 10, 20, 30, 40 and 50% PFM protein replaced by TFM protein. Proximate composition of crude protein, crude lipid and gross energy ranged from 46.45% to 49.39%, 12.30% to 12.91% and 472.55 to 491.80kcal/g, respectively. Each diet was randomly assigned to triplicate groups of 12 fishes per tank (initial body weight 1.34 g). Fish were maintained in a recirculation seawater system. Water temperature and salinity range from 26 to 32⁰C and 30 to 33ppt, respectively. Fish were hand-fed three times daily to apparently satiation for 8 weeks and weighted every two weeks. The termination of the experiment, there were no significant differences in final weight, weight gain of grouper fed diets containing 0, 10, 20 and 30% TFM protein. The weight gains of grouper fed diets containing 40% TFM protein (WG 1300.54 %) and 50% TFM protein (WG 1153.69%) were significantly lower than those of grouper fed diets containing 0, 10, 20 and 30% PFM protein. Furthermore, there were no significance differences in survival. The results indicated that the growth performance decreased with increasing TFM. Therefore, based on this experiment, up to 30% of dietary fish meal protein can be replaced by using TFM without an adverse effect on growth performance of grouper.
For second experiment, this study investigated the effects of dietary replacement of fish meal with protorsan meal on the growth performance of orange-spotted grouper Epinephelus coioides. Five isonitrogenous (45% crude protein) and isolipidic (11% crude lipid) were formulated to include 0, 5, 10, 15 and 20% protorsan meal protein (PSM) to replace fish meal protein. This experiment was carried out in a completely randomized design with three replicate for each treatment diet and each replicate contained 7 fish (average initial body weight 1.87 g) for six weeks. Fish were maintained in a recirculation seawater system and water temperature and salinity were kept from 27 to 32ºC and 30 to 33ppt, respectively. Fish were hand fed to satiation three times daily and weighed every three weeks. Fish fed diet included 5% PSM had significantly higher weight gain percentage than those fed diets included 15 and 20% PSM. However, there were no significantly different weight gain percentage of fish fed diets containing 0, 5 and 10% PSM. Grouper fed diet included 20% PSM had the significantly lowest weight gain percentage among treatment groups. Feed conversion ratio (FCR) of grouper fed diets containing 0 and 5% PSM was significantly lower than that of grouper fed other treatment diets. There was no significantly different in survival among treatment groups and ranged from 95 to 100%. This study indicated that diet can be included 5% PSM to replace fish meal protein and showed no adverse effect on growth performance of grouper.
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