Rapid Circulating Tumor Cells Diagnosis on Self-Assembled Cell Array (SACA) Chip by In-Parallel Image Analysis and In-Situ Cell Capture
碩士 === 國立清華大學 === 工程與系統科學系 === 104 === Malignant tumor is the first of top ten death causes in Taiwan. The difficulty is not only the treatment of malignant tumor eradication, and it is more difficult to prevent cancer metastasis. It makes treatment time stretched and hard. Circulating tumor cells (...
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| Format: | Others |
| Language: | zh-TW |
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2016
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| Online Access: | http://ndltd.ncl.edu.tw/handle/23043115165151537473 |
| Summary: | 碩士 === 國立清華大學 === 工程與系統科學系 === 104 === Malignant tumor is the first of top ten death causes in Taiwan. The difficulty is not only the treatment of malignant tumor eradication, and it is more difficult to prevent cancer metastasis. It makes treatment time stretched and hard. Circulating tumor cells (CTCs) are generated when tumor metastasis occurs. Recent research represents the severity of disease is related to the amounts of circulating tumor cells. So it is an important research direction which finds out and analysis circulating tumor cells for the clinical study.
This report uses a fluid feature for detecting CTCs. Self –Assembled Cell Array Chip makes up with SU-8 photoresist on a glass substrate. Cells produced by flow of liquid and gravity to form a high density monolayer array after fluorescent calibration. In the end CTCs can be count by the fluorescence microscope. It has IRB cooperated plan with hospital and allowed to get colorectal cancer and breast cancer patient blood sample to do the clinical research. The result of SACA Chip will be compared with flow cytometry and IsoFlux.
This report provides an inexpensive and simple way to make chip and the process costs less than ten minutes. It also builds up the automatic imaging and in-situ cell capturing process to shorten the process time and isolate CTCs after process.
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