| Summary: | 博士 === 國立中山大學 === 海洋生物科技博士學位學程 === 104 === This thesis presents the exploration of nanoparticles in the use of biological application. Here in this work various nanoparticles are employed like Graphene oxide and Gold nanorods use for photothermal therapy. Carbon dots use as a matrix and drug delivery vehicle.
Graphene oxide (GO) is a close derivative of graphene has unlocked many pivotal steps in drug delivery due to their inherent biocompatibility, excellent drug loading capacity, antibacterial, antifungal and high water solubility. we have conjugated them with gold nanorods (GNRs) using in situ synthesis of GO@GNRs via seed mediated method. To the above conjugate, Doxorubicin (DOX) was attached at ambient temperature (28±2°C). The enhancement in NIR induced drug release and photothermal property was observed which indicates that the fGO@GNRs-DOX method is an ideal choice for chemotherapy and photothermal therapy simultaneously. Delivery of therapeutic moieties using water soluble Carbon dots (C-dots) has been pivotal to control the release of the drugs under physiological condition due to their high biocompatibility. Controlled Dopamine hydrochloride (DA), a potential neurotransmitter using C-dots as carriers is studied in the present work, in order to highlight its potential to deliver drugs related with neurological disorders such as Alzheimer’s and Parkinson’s disease. In order to understand the impact of the C-dots-DA conjugate under physiological conditions, Nero 2A cells were taken under consideration.
Photothermal treatment of graphene oxide (GO) for antibacterial, antifungal and controlling the wound infection treatment using near infrared laser Nd-YAG (1064 nm) were reported. Various pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus) and fungal (Saccharomyces cerevisiae and Candida Utilis) were investigated. The Cytotoxicity was measured using the proteomic analysis, optical density (OD600), standard micro dilution procedures, TEM and Epifluorescence microscopy. The laser mediated surface activation of GO was achieved for efficient antifungal and antibacterial therapeutic strategy. GO provided unassailable effects and wide applicability.
Wound infection treatment is one of the most challenging problems to be addressed in infectiously microbiological treatment. This is mainly due to the pathogen’s ability for fast mutation and generating severely antibiotic resistance to antimicrobial treatment. Therefore, we have proposed a novel method by using gold nanorods (Au NRs) to assist the Nd-YAG laser (1064 nm) for photothermal killing pathogenic bacteria (Pseudomonas aeruginosa) for directly healing the wound infection on the (albino) mice. The current approach can be used to control severe skin infections from antibiotic resistant pathogens in wounds.
Carbon dots (C-dots) exhibit strong absorbance in the UV (220-350nm) range, which was exploited to transfer the energy from N2 laser (337 nm) of Matrix-assisted laser desorption/ionization-Mass Spectroscopy (MALDI-MS) to analytes for their rapid detection. Due to this strong feature and extremely small size (2- 4 nm), they were used to enhance the signal intensity of MALDI-MS peaks of low molecular weight biomarkers in serum. In this study, we utilized the extraordinary property of C-dots as a matrix for the detection of Serotonin (Sr), Glutamic Acid (GA) and Dopamine Hydrochloride (DA) by using MALDI-MS.
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