| Summary: | 博士 === 國立中山大學 === 海洋生物科技博士學位學程 === 104 === The prime interest of the thesis involves biomedical and environmental applications of
nanoparticles including sensors for bacteria and therapeutic agents present in the biological fluid and metal contaminants in environmental samples. This thesis was also focused on fabrication of anti-bacterial nanoparticles (calcium oxide nanoparticles) and fluorescent carbon dots using waste sea food as precursors.
The novel nanomaterials such as fluorescent carbon dots are investigated for their potential
application such as possible matrix in matrix assisted laser/desorption ionization mass
spectrometry (MALDI MS) for quantification of non-steroidal anti-inflammatory drug, and
mefenamic acid in serum. We found that the carbon dots can play an important role in the detection of low molecular weight compounds. In comparison with conventional matrix such as 2,5-dihydroxy benzoic acid, carbon dots were found to be an outstanding matrix to avoid background signals and fragmentation of the mefenamic acid signals. Furthermore, the developed methods were applied for the detection of mefenamic acid biological fluid such as serum.
The efficiency and function of the nanoparticles depend on their shape, size and surface modified
properties. To fine tune the nanoparticles, various synthetic methods were applied such as
hydrothermal pyrolysis, salvo-thermolytic including both top down and bottom up approaches. A novel method had been developed for the synthesis of calcium oxide nanoparticles from marine sea food waste by green synthesis method. Further, the antimicrobial effect of calcium oxide nanoparticles was also studied to against gram positive and gram-negative bacteria. We found that minimum inhibitory concentration of the calcium oxide nanoparticles was 10 μg /mL for micro-organisms by optical density, disk diffusion and MALDI-MS. Highly fluorescent carbon
dots were also synthesized as a byproduct of food waste recycling during the synthesis of calcium oxide nanoparticles.
We further explored these carbon dots as probes for a fluorescent Cu2+ ions sensing application.
This fluroscence sensing platform exhibited excellent selectivity and sensitivity toward Cu2+ ions with detection limit as low as 5 nM. The practical application of this sensing platform for the determination of Cu2+ ions in the seawater samples was also successfully demonstrated. This forms the fundamental basis of development of sustainable green technologies for exploiting organic and inorganic waste for nanoparticles synthesis. The method was tuned to enhance the yield of nanoparticles in order to make it to become the industrially powerful method.
A metal oxide nanoparticle such as zinc oxide was synthesized for separation of bacterial cells
from water samples using liquid phase micro-extraction. Zinc oxide nanoparticles were modified with polymethyl methacrylate to make the surface of zinc oxide completely hydrophobic to
clutch bacterial cells. The separated bacterial cells were identified using MALDI MS. The results
indicated that the above approach is a simple, rapid and efficient micro extraction technique for the analysis of pathogenic bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa.
The method was validated by the analysis of real samples, such as tap and drinking water.
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