Studies on the Secondary Metabolites from the Soft Corals Sinularia leptoclados, Lobophytum michaelae, Sarcophyton trocheliophorum, and Sinularia flexibilis

• Main Authors: Yao-Li Yun, 雲曜立
• Other Authors: Chang-Yih Duh
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/97678087985410567431

碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 104 === Natural product scientists have reported many secondary metabolites containing sesquiterpenoids and cembranoids from soft corals Lobophytum spp., Sacrophyton spp., and Sinularia spp., and further search for new compounds with biological activity. In this report, we focused on marine natural product extraction and separation studies, purified by various chromatographic techniques, isolating thirteen compounds from the soft corals Sinularia leptoclados, Sacrophyton trocheliophorum, Lobophytum michaelae and Sinularia flexibilis of Green Island, Gueishan Island and Pratas Island. Thirteen compounds (1−13), including one new sesquiterpene compound (1) and four new cembrenes (2−5), along with eight known compounds, epoxycembrene (6), diepoxycembrene B (7), diepoxycembrene A (8), 5-dehydrosinulariolide (9), (1R, 13S, 12S, 9S, 8R, 5S, 4R)-9-Acetoxy-5, 8: 12, 13-diepoxy-cembra-15(17)-en-16,4-olide (10), querciformolide A (11), sinulariolide (12), and sinulariolone (13), have been isolated from organic extracts of soft corals Lobophytum michaelae, Sacrophyton trocheliophorum and Sinularia Leptoclados. The structures of isolates 1-13 were elucidated by spectroscopic evidences (1D, 2D NMR, Mass, IR, UV, and optical rotation) and compared with the spectral data of known compounds. The cytotoxicity of these isolates against A-549 (human lung epithelial carcinoma) and HT-29 (human colon adenocarcinoma), and were found compound 2, 4, 6 and 9 had cell activity (1.29−1.76 μg/ mL) .