Investigation of dioxin-like and estrogen-like effects in the elementary schools and kindergartens and exploration of their potential contaminated sources

• Main Authors: Huang, Shin-Ming, 黃憲民
• Other Authors: Chao, How-Ran
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/54905154074433542707

碩士 === 國立屏東科技大學 === 環境工程與科學系所 === 104 === Several studies reported that many endocrine disrupter compounds (EDCs) including dioxin and dioxin-like compounds and estrogen and estrogen-like compounds occur mainly in the indoor dust. Chemical analysis (HRGC/HRMS) is the major method for the investigation of EDCs levels in environmental samples. Although chemical analysis has high sensitivity and accuracy, it is mainly costly and was time-consuming. Therefore, the reporter gene assay was established and widely used to survey EDCs levels in the recent year. The aim of this study is to use two reporter gene assays (Ad-DR bioassay and T47D-KBluc assay) to investigate the dioxin toxic equivalency (TEQ) and estrogenic toxic equivalency (EEQ) in twenty elementary schools and kindergartens floor dusts from southern Taiwan, and evaluate the health risk and potential sources. The Soxhlet extraction and cleanup procedures were used in the present study which followed Taiwan Environmental Protection Administration (EPA) standard method (NIEA S103.61C). In this study, the concentration of TEQs and EEQs are ranged 0.798-16.3 (mean:5.14) ng BEQ /kg d.w. and <LOD-5.85 (mean:0.782) ng EEQ/kg d.w., respectively. For the statistical analysis, our result showed significant correlation between concentration of TEQ and schools’ distance from Pingtung industrial area (p=0.024). In addition, the TEQ levels in school’s neighboring educational and business area was significantly higher than neighboring agricultural area (p =0.005), and also observed association with high traffic (p=0.011).The principal component analysis (PCA) results indicated that Pingtung industrial area and Neipu industrial are two potential source, and may affect TEQ levels in elementary school and kindergarten dusts. For health risk assessment, the average TEQ daily intake (DI) of children via ingestion elementary school and kindergarten dust was 1.98×10-12 mg WHO2005TEQ/kg d.w/day, whereas the Hazard quotient (HQ) and Cancer risk (R) were 1.62×10-8 and 1.15×10-9, respectively. Both of these results were under the critical thresholds (HQ=1.00 and R= 1.00 × 10-6).