Protective Effect of Chlorogenic Acid in Macrophages and DSS-Induced Acute Colitis in Mice Model

• Main Authors: Chang, Tsung-Yu, 張宗佑
• Other Authors: Liao, Ming-Hui
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/17542524171450783834

碩士 === 國立屏東科技大學 === 獸醫學系所 === 104 === Chlorogenic acid (CGA) , has been reported to improve the index of inflammatory bowel disease (IBD) . CGA is one of the most abundant polyphenols in the human diet and is suggested to be a potential anti-atherosclerotic agent due to its proposed hypolipidemic, anti-inflammatory and anti-oxidative properties. To analyze the possible protective role of CGA, macrophage cell model and mice model were set up. The aim of this study was to evaluate the effect of chlorogenic acid on Lipopolysaccharide (LPS) stimulated macrophage and DSS-induced acute colitis in mice. Inhibition of CGA on the translocation of transcription factor NF-κB in macrophage was observed by using confocal microscope. Moreover, the histological findings showed that CGA treatment can prevent inflammatory cell infiltration and colon damage. We postulated that CGA can prevent intestinal damage and decrease inflammatory cytokines in mice with DSS-induced colitis. Keywords: Chlorogenic acid, Colitis, Mice model, NF-κB