Development of a rapid detection method of Fusarium oxysporum f. sp. cubense via surface-enhanced Raman scattering

• Main Authors: Lin, Yi-Jia, 林依佳
• Other Authors: Lin, Ying-Hong
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/00447474143482961453

碩士 === 國立屏東科技大學 === 植物醫學系所 === 104 === Fusarium wilt of banana (FWB) caused by Fusarium oxysporum f. sp. cubense (Foc) has been considered as one of the most important diseases of banana. In order to reduce the impact of FWB outbreak, development of a specific, fast and on-site detection tool for Foc is important. Raman spectroscopy, an emerging laser-based technology based on Raman scattering, has been used for qualitative characterization of biological tissues such as foodborne pathogens, cancer cell, and melamine. The method should be sensitive, reproducible, and rapid, and could potentially be a detection tool for on-site detection of Foc. The purpose of this study is to develop a spectral diagnostic method based on Surface-enhanced Raman spectroscopy (SERS) for rapid detection of F. oxysporum f. sp. cubense and diagnosis of Foc-infected banana tissues. In this study, the Raman fingerprints of hyphae and conidia of Foc were established. The author found 9, 8, and 6 Raman-barcodes for spectral the identification of macroconidia, microconidia, and hyphae, respectively. Based on the Foc-specific fingerprints, the Raman spectroscopy assay could be used to detect Foc. Moreover, this approach was be used to detect the banana samples of varyingly symptomatic Foc-infected pseudostems. And the detection rate of no symptoms samples by Raman spectroscopy was 73%. The novel method will be applied to detect the Foc chlamydospore in the field. The surface-enhanced Raman spectroscopy assay developed in this study provides an alternative method in the detection of Foc. The spectral diagnostic method has the potential to serve as a rapid, specific, and sensitive platform for the routine on-site detection of Foc. The assay should be a powerful tool for reducing the dissemination of the pathogen and the economic impacts of FWB on the banana industry.