薑黃素對雄性大鼠暴露鄰苯二甲酸二(2-乙基己基)酯誘發精子傷害之保護效應

• Main Authors: Yi-peng Jiang, 江逸鵬
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/05182798439960671172

碩士 === 國立高雄第一科技大學 === 環境與安全衛生工程研究所 === 104 === Background: Di (2-ethylhexyl) phthalate (DEHP) is the most crucial phthalate added to polyvinyl chloride as a plasticizer. Many studies indicated that DEHP was associated with developmental, reproductive and hepatic toxicity in animals and humans. Curcumin (CUR) is a phytochemical derived from rhizome of curcuma longa, present in the curry spice. CUR has exhibited anti-inflammatory, anti-infective and antioxidant properties. Methods: Experiment 1: Male 42-postnatal day (PND)-old Sprague Dawley (SD) rats were randomly divided into control, 1 mg/kg, 2 mg/kg, and 8 mg/kg groups to establish the dose-response assessment of CUR. Experiment 2: Animals were divided into control, CUR group (2 mg/kg), DEHP (1000 mg/kg), and CUR+DEHP (CUR 2 mg/kg + DEHP 1000 mg/kg) groups to evaluate the protective effects of CUR on DEHP-induced sperm dysfunctions. The change of body weight and amount of drinking water were recorded from PND 42 to PND 105; at the end of the experiment, all rats were euthanized and their testes and sperm functions were analyzed. Results: In experiment 1, the sperm super oxide anion levels of rats in the CUR 2 mg/kg group significantly decreased. In experiment 2, we found relative testis and epididymis weights in the DEHP group significantly decreased compared with those of control, CUR, and CUR + DEHP groups. The sperm super oxide anion levels of in the CUR and CUR+DEHP groups significantly decreased compared with those of their controls, respectively. Sperm chromatin structure analysis was used and we found that the intensity of sperm DNA damage in DEHP group was significantly increased. Meanwhile, sperm DNA damage in CUR+DEHP group was not significantly affected compared with control group. Moreover, diploid phase rate of testicular cells was significant increased and the percentage of round spermatids was significant decreased in DEHP group compared with control group, respectively. Conclusion: This study provides suggestive evidence of the protective role of CUR on DEHP-induced oxidative stress and sperm chromatin DNA damage.