| Summary: | 碩士 === 國立東華大學 === 生命科學系 === 104 === Group B Streptococcus, GBS, also known as Streptococcus agalactiae, is common in female urinary, reproductive systems, or intestinal tract. It may cause puerperal fever during pregnancy and develops into maternal sepsis. Under the exposure to the source of infection, the fetus or newborn suffers neonatal pneumonia, meningitis, sepsis, and even death. It is an extremely valued bacterium. Despite the existence of rapid methods for detection of GBS, including bacterial antigen detection and molecular biotechnology, they are applicable to large-scale epidemiological survey only. Most of these methods have not been widely used in clinical practice. It is still relied on traditional methods to detect GBS in clinical practice. It takes 4～5 days from sample collection, enrichment, training, isolation, and identification. This study has been aiming at the improvement of the traditional methods in order to simplify the procedure, shorten time of execution, and raise accuracy of detection of GBS, including the γ hemolysis B streptococci, and the average detection rate increased to 22.16%.
Some epidemiological studies indicated that capsular antigen serotypes Ia, III, and V are most closely connected with the disease. Therefore, this study also investigated the isolated capsular serotype distribution of group B streptococcus from an eastern regional teaching hospital in Taiwan. Combined with the antibiotic susceptibility test, analysis among various capsular serotypes for susceptibility of different antibiotic helps physicians in making a diagnosis in clinical practice. According to specific antibiotics can be chosen in treatment for reducing neonatal group B streptococcus infection based on pathogenic severity of different serotypes. The results showed that the susceptibility testing, a total of 102 GBS isolates were susceptible to penicillin and vancomycin, it was also not found in resistant strains. Resistance to erythromycin and clindamycin was found in 45.1% and 49.0%. Serotype III (27.5%) is the most common serotypes, followed by V (23.5%), Ib (15.7%).
|
|---|
