| Summary: | 碩士 === 國立嘉義大學 === 生化科技學系研究所 === 104 === Osteoarthritis (OA), a disabling joint disease, occurs more frequently in people at the age of 65 or older. OA is characterized by synovial inflammation and progressive cartilage damage. Curcumin (Cur, diferuloylmethane) is a non-water-soluble polyphenol compound that has anti-inflammatory potential. Curcumin have beneficial effects on osteoarthritis treatment and have been approved for relieving osteoarthritis pain. However, curcumin have shown low solubility and poor bioavailability in previous studies. In order to increase the therapeutic effect of curcumin, we used soybean phosphatidylcholine to encapsulate curcumin for liposomal formation. Cur-loaded liposomes have been characterized in particle size, encapsulation efficiency, zeta potential, polydispersity index, liposomal stability and cellular uptake. In here, we evaluated the effect of lipid composition and drug concentration of curcumin-loaded liposomes on Interleukin-1β induced human chondrocytes SW-1353.
The results show that there are about 60% entrapment efficiency of Cur in liposomes and particle sizes are stable after liposome formation. Liposome formation can reduce the cytotoxicity of curcumin in SW-1353 chondrocytes, and increase cellular uptake. With IL-1β stimulation, Cur-loaded liposomes can successfully down-regulate the expression of inflammation markers such as IL-6 and IL-8 and then suppress MMP-1 and MMP-3 expression on SW-1353 chondrocytes. In this study, we observed that Cur can be encapsulated in liposomes successfully and which can reduce MMPs and cytokines in IL-1β stimulated SW-1353 chondrocytes. Moreover, Cur-loaded liposomes suppressed IL-1β-induced GAG release in chondrocytes. In this study, we investigated the impact of different concentrations lipids of liposomes on adipogenic differentiation of SW-1353 chondrocytes. SW-1353 chondrocytes were incubated in adipogenic differentiation medium (ADM) with different liposomal concentrations, and compare their effects on lipids accumulation and adipogenic differentiation. Our results demonstrated that ADM can induce adipogenic differentiation and lipid accumulation in SW-1353 chondrocytes but Cur-loaded liposomes may reduce lipid accumulation in ADM-treated SW-1353 chondrocytes. Therefore, Cur-loaded liposomes can suppressed inflammation responses, MMP expression, GAG release and lipid accumulation in a cell model of OA.
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