Develop the miR-524-5p by in situ hybridization as an ancillary biomarker in melanoma diagnosis

• Main Authors: Sung-Nine Wang, 王松年
• Other Authors: Nian-Han Ma
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/84412961958914717900

碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 104 === Our previous study demonstrated that over-expression of miR-524-5p suppresses cells proliferation, migration and tumor growth of melanoma in mice. In addition, miR-524-5p plays an important function in regulating MAPK/ERK signaling through directly suppressing BRAF and ERK2 in melanoma cells. In previous study, the expression of miR-524-5p is inversely associated with the activity of the MAPK/ERK pathway in melanoma cells. However, the expression level of miR-524-5p in melanoma patients is unknown and if there are similar inversely expression pattern between MAPK pathway activity and miR-524-5p in human tissue. We investigated the expression of miR-524-5p and MAPK activity using total 58 tissues from melanoma and nevus from Taiwan patients and total 99 melanoma and nevus tissues from commercial tissue array. The expression of miR-524-5p was detected by in situ hybridization (ISH) and the activity of MAPK/ERK pathway (assessed by phosph-MEK) and BRAF expression were measured by immunohistochemistry (IHC). These results could assist to determine the pathology for melanoma or further applied in the biomarker of melanoma diagnosis.