| Summary: | 碩士 === 國立中央大學 === 化學工程與材料工程學系 === 104 === Global warming has become the biggest problem currently facing the earth, which accounted for a large part of the reason for the extensive use of fossil fuels. In order to reduce the use of fossil fuels, and began to study other alternative sources of energy, so the rise of renewable biofuels. Biofuel is one of the main use of yeast fermentation production of ethanol.
In recent years, the fuel ethanol replaces gasoline that has been extensively used and attention. Yeast strains of Saccharomyces cerevisiae have been extensively studied for bio-ethanol. Previous studies have indicated that the S. cerevisiae fermentation to produce ethanol, the volume of ethanol in the fermentation broth reached 4 vol% to 10 vol%, ethanol will in turn on cell growth inhibition, leading to S. cerevisiae was unable to produce ethanol, and even death. Saccharomyces cerevisiae BCRC 21812 of ethanol tolerance was 8.87 vol% in this study.
In order to avoid the occurrence of tolerance, a novel bioreactor has been developed, continuous extractive fermentation system by polydimethylsiloxane (PDMS) sponge. PDMS sponge is extracting agent, and the adsorption and desorption of ethanol is extruded by sponge. This system can remove more than 50% of ethanol in ethanol solution. After ten successive extraction, the medium was increased the total amount of ethanol by 21.07% compared to batch.
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