Continuous Butanol Production by using PDMS Sponge Extractive Fermentation

• Main Authors: Yang Chun-Hsing, 楊淳行
• Other Authors: Chin-Hang Shu
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/81085341876177756660

碩士 === 國立中央大學 === 化學工程與材料工程學系 === 104 === In this research, we extract butanol from the continuous fermentation of Clostridium acetobutylicum with Polydimethylsiloxane (PDMS) sponge , and discuss its effect and feasibility. As the concentration of butanol reach about 10 gL-1 in ABE fermentation, Clostridium acetobutylicum will be inhibited by its product, butanol, and lose its activation. It will become into a state of endospore and stop to product butanol. Thus, we will reach the target of promoting the productivity of butanol by circulating the broth into PDMS sponge system by peristaltic Pump and refresh the broth in suitable moment. PDMS sponge is used to extract the inhibitor in this research. When the fermentation reach 48 hours, it will be extracted butanol every 12 hours. The volume of working fluid of the broth is 150 mL. It will be inject into the system including 10 g PDMS sponge for 15 minutes by peristaltic Pump and reflow. It will be refresh the 50 mL broth into liquid medium in same volume. In contrast to the control, the total productivity of butanol is promoted from 1.725 to 7.89 g. The fermentation period is expanded from 108 to 192 hours. Because of PDMS sponge and the continue extractive fermentation, It will not only be slow the losing of the activation down, but also cut the inhibit effect off. And the total productivity of butanol is increased. Therefore, the process feasibility of using PDMS hydrophobic sponge as an extractant, is worth in-depth study.