| Summary: | 碩士 === 國立中央大學 === 化學工程與材料工程學系 === 104 === In recent years, various biomarker detection methods have been invented and studied. Optical, mass, electrical and etc… biosensors are common for monitoring DNA, RNA, protein, and so on. Among them, Silicon nanowire field effect transistor (SiNW-FET) is the one that has been extensively studied and developed due to its distinguished advantages over the other biosensors. SiNW-FET is ultrasensitive, selective, real-time and label-free detection platform. Because of these features, SiNW-FET has a great potential in the future of clinical diagnostics. In the current process of SiNW-FET immobilization, we mostly use all area modification. However, if we use this method to immobilize bioreceptors on SiNW-FET, the device can detect only one kind of biomarker. To solve this problem, we develop a technique called “multi-point immobilization system”. Multi-point immobilization system can overcome the above-described problems because this system can spot many kinds of bioreceptor solution on each device. If there are more bioreceptor probes on SiNW-FET, we can detect more categories of biomarker targets on same device. Saving costs and experimental time are comparative advantage with all area modification. In this research, first we investigate the effects of salt ion concentration on hybridizing. Then we explore the best ratio of glycerol in probe solution and optimal probe concentration in multi-point immobilization system. Finally we try to immobilize different probe on same device to measure electrical signal.
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