| Summary: | 碩士 === 國立交通大學 === 環境工程系所 === 104 === In this study, a Shaker-Fluidized bed-Atomizer disperser (SFA) powder dispersion system consist of an atomizer, powder storage container, supporting base and orbital shaker with 0.5 L/min dispersion flow rate and 35 psi compressed air was used to generate long-term stable concentration and well-dispersed multi-wall carbon nanotube (MWCNT) and purified multi-wall carbon nanotube (P-MWCNT).
The physicochemical properties both of MWCNT and P-MWCNT dispersed by SFA such as number distribution, mass distribution, morphology, real-time mass concentration and surface area concentration were measured before animal inhalation exposure test. In addition, the purity and functional groups of MWCNT and P-MWCNT were also measured. The result of mass distribution shows bimodal distribution with 0.26、3.05 µm and 0.18、1.28 µm of MMAD for MWCNT and P-MWCNT, respectively. Also the carbonyl group (-COOH) appeared on the surface of P-MWCNT after purification.
To investigate biological effects of MWCNT and P-MWCNT on animal, the SFA dispersion system was combined with Nanoparticle Exposure Chamber System (NECS) in NHRI to conduct whole-body inhalation exposure at high dose MWCNT (MWCNT-HD), low dose MWCNT (MWCNT-LD) and low dose P-MWCNT (P-MWCNT-LD) exposure groups for 4 hour per day and 5 days per test.
Results indicated that the severity of pulmonary function impairment from high to low was MWCNT-HD, MWCNT-LD and P-MWCNT-LD. The results of histopathology also showed obvious inflammation, alveolar wall thicken and interstitial pneumonia-like histopathological outcomes caused by MWCNT-HD exposure. Due to better dispersion, P-MWCNT showed the highest deposition amounts in lung but did not present the most serious biological responses. The study proposed protein corona adsorbed on P-MWCNT eliminating acute phase biological responses and the short-term observation after one-week exposure was not able to present chronic effects.
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