Design and Synthesis of Mercury(II) and Hypochlorous acid Fluorescent Chemosensors and their Applications in Living Cells

• Main Authors: Liu, Yen-Yu, 劉彥佑
• Other Authors: Wu, Shu-Pao
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/42824538746720576295

碩士 === 國立交通大學 === 應用化學系碩博士班 === 104 === In this thesis, pyrene-based and coumarin-based chemsensors, PES and CHT, were designed to detect Hg2+ and hypochlorous acid. PES contains a bis(ethylsulfane) group as a reaction moiety for Hg2+, and exhibits good selectivity and sensitivity over other metals. PES shows weak fluorescence with a quantum yield (Φ=0.01), and 27 fold blue fluorescence enhancement (Φ = 0.27) is produced upon removal of bis(ethylsulfane) by Hg2+. The intensity of PES in the presence of different Hg2+ concentration shows a good linearity with a detection limit of 55 nM. Confocal fluorescence microscopy imaging using RAW 264.7 cells shows that PES can be an effective fluorescent probe for Hg2+ in living cells. In second part, CHT uses hydrazinecarbothioamide group as recognition moiety for HOCl detection, and exhibits good selectivity and sensitivity over other ROS and RNS. The detection limit of CHT for HOCl is 0.82 μM and displays good sensitivity in buffer solution in the pH value of 6.0 to 10.0. Confocal fluorescence microscopy imaging using RAW 264.7 cells shows that CHT could be an efficient fluorescent probe.