Development of a lab-on-a-chip system for label-free detection by integrating photonic crystal and electrowetting-on-dielectric digital microfluidic

• Main Authors: Fu, Ching-Su, 傅敬書
• Other Authors: Huang, Chen-Shen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/zj7x38

碩士 === 國立交通大學 === 機械工程系所 === 104 === Traditional immunoassay experiment including antibody immobilized, sample dilution, reagent mixing, marking, regenerate, washing etc, is tedious and highly repetitive and needs different equipment to operate each step. The concept of Lab-on-a-chip (LOC) attempts to integrate all experimental steps into one chip. . Using digital microfluidic system as an operating platform has many advantages: easy manufacture, work without pumps or valves, no blocking issue, less waste of samples, and programmable computer controlled automatic progress. These advantages are consistent with the needs of biomedical research. In clinical diagnosis, immunoassay based on fluorescence detection is the most common technique for disease detection; however, it requires to conjugate fluorophores with biomolecules. Hence, it is a time-consuming process and fluorescence quenching could be a potential issue. This study develops a LOC system which combines electrowetting-on-dielectric (EWOD)chip and photonic crystal (PC) chip for label-free detection. Through reflection measurement, we accomplish two-fold serial dilution and successfully measured different concentrations of sucrose solutions. The sensitivity of our device is 44 nm/RIU.