Integration of Photonic Crystal with Microfluidic Chip as Biosensing Platform

• Main Authors: Tu,Yi-Kai, 涂逸凱
• Other Authors: Huang, Cheng-Sheng
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/59690450204753572401

碩士 === 國立交通大學 === 機械工程系所 === 104 === The goal of this project is to develop a novel Lab-on-a-chip (LOC) detection system. The proposed system consists of a microfluidic subsystem and a label-free (LF) detection subsystem. The system can facilitate sample preparation and provide immediate detections. The microfluidic subsystem consists of several components including two liquid inlet which can inject the samples and reagents, and filters for cell debris purification after lysis. All these components will be fabricated on a silicon chip simultaneously. Regarding the detection subsystem, we developed a sensitive photonic crystal (PC) biosensor for LF detection of proteins. The PC functions as an optical filter where only a particular combination of wavelength/illumination angle can excite the structure resonance, resulting in a strong reflection, while other combinations of wavelengths and illumination angles are transmitted through. Through monitoring the shift of the reflected wavelength, the concentration of the analyte can be determined. The designed LOC system was demonstrated to detect the Beta-actin proteins inside the cell and simultaneously to filter the cell debris. The result indicates the detection limit of 160 ng/ml can be achieved without any prefiltering procedure.