| Summary: | 碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 104 === Metastasis is associated with 90 % of cancer-related deaths and circulating tumor cells (CTCs) play an important role as an intermediate during the metastatic invasion. Targeting CTCs when they are transiting in the blood circulation could disrupt cancer dissemination and reduce the probability of cancer death. Herein, we introduce a magnetic photosensitive nanocarrier, gold nanocages-conjugated thiol-magnetic nanocarriers (GTMNCs) to trap and destroy cancer cells in situ in flow. Gold nanocages (AuNCs) are able to induce hyperthermia which results in cancer cells necrosis under 808 nm irradiation and sensitize singlet oxygen (1O2) formation to activate apoptosis program upon NIR illumination (940 nm LED, 915 nm laser). The temperature of the suspension of GTMNCs raised to more than 50 oC under 808 nm laser light. 1O2 phosphorescence emission and singlet oxygen sensor green (SOSG) fluorescent probe were used to confirm the 1O2 generation upon NIR illumination. As GTMNCs were functionalized with anti-epithelial cell adhesion molecule (EpCAM) antibody to specifically target mouse breast cancer cell (4T1), in vitro experiments demonstrated 9-fold increase in intracellular ROS levels by quantifying SOSG and dihydroethidium (DHE) fluorescence. Scavengers were also used as negative control. Cytotoxicity assay indicates that GTMNCs are high biocompatibility in dark until photoirradiation for performing photothermal therapy (PTT) and photodynamic therapy (PDT). Further magnetic enrichment of 4T1 cells and simultaneous photodestruction in the microfluidic channel using the GTMNCs proved a potential methodology of phototherapy for CTCs removal.
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