Integration of Blood cell separation and ZnO Nanowire Microfluidic Devices as Biosensors

• Main Authors: Wang, Chun-Ying, 王俊穎
• Other Authors: Sheu, Jeng-Tzong
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/78ze28

碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 104 === In this thesis, a biosensing platform integrating a blood cell separation microfluidic device and a zinc oxide nanowires fluore-sensing structure was investigated and demonstrated for prostate specific antigen (PSA) detection. Blood cells usually cause signal interference and decrease efficiency of antigen/antibody reaction in the whole blood assay. Therefore, an effective microfluidic channel chamber for blood cell separation was designed via the help of COMSOL simulation. An optimal dimension of microfluidic channel chamber with 25000 μm in width, 8000 μm in height and 200 μm in thickness can reach a blood cell separation rate of 98.9%. The sharp ZnO nanowire (NW) with cone-like structure increases the surface area and fluidity enhances the capture efficiency of target molecules. Therefore, sharp ZnO NWs were grown in the microfluidic channels to enhance the dynamic range and the sensitivity. Prior to sensing, 3-aminopropyltrimethoxysilane (APTMS), disuccinimidyl suberate (DSS) and PSA antibody 8A6 were modified on the ZnO nanowires via the self-assembly process. Whole blood samples spiked with various concentrations of PSA antigen plus fluorescent PSA antibody 5A6 were adopted to characterize the limit of detection from 1 ng/mL to 10 ng/mL. This platform also presented a capability to distinguish PSA with 10 ng/mL, 5 ng/mL and 1 ng/mL concentrations in blood. With a very simple structure and a need of only 40 μL sample, this prototype platform provides promising results as a future portable Lab-on-a-chip.