Three-dimensional cell culture of stem cells in peptide hydrogel

• Main Authors: Wu,Fang-Yi, 吳芳誼
• Other Authors: Lin,Hsin Chieh
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/y78w69

碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 104 === Self-assembly of peptide-based materials from rationally designed of the chemical structures have received great attention in recent years. Self-assembly provides routes to a range of materials with regular structures. In this study, we have synthesized a series of peptide-based low molecular weight hydrogelators. This thesis has two-fold part. In the first part, we design a new amphiphilic hydrogelator (Ben-FF, 4-F-Ben-FF, 2,4,6-F-Ben-FF and PFB-FF) which forms stable hydrogel in neutral pH conditions. Different number of fluorine replace the hydrogen on aromatic ring effect different mechanical property also the intermolecular π-π interactions can be enhanced, indicating the remarkable effect of fluorine on supramolecular hydrogelation. In the second part, the additional functional sequence conjugated at the terminal of phenylacetic acid and pentafluorophenylacetic acid. We development materials of a self-assembly supramolecular hydrogel which can provide an environment for cell proliferation and induce human mensenchymal stem cells (hMSCs) differentiate to neuron lineages. We hope to understand the interaction between cells and biomimetic materials then utilize its properties to be controlled stem cell differentiation thus damaged tissue can be replaced. The results shows that extended functional motif can enhanced hMSCs toward neuronal differentiation by qPCR and immunofluorescent staining. As for in vitro experiments, the relative low cytotoxity of peptide hydrogel was examined by live/dead assay. The results demonstrated that the hMSCs survived well with 3D culture in peptide hydrogel. A tunable substrate stiffness that would induce hMSCs specifically toward neuronal differentiation. It is belived that our supermolecular hydrogel can be promising material for tissue engineering application.