Elucidation of medicinal herbs for inhibitory effects against dengue virus type-2 (DENV-2) NS3 helicase domain ATPase activity

• Main Authors: Lim, Swee-Teng, 林瑞婷
• Other Authors: Lin, Tiao-Yin
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/61429883987225673045

碩士 === 國立交通大學 === 生物資訊及系統生物研究所 === 104 === Dengue virus (DENV) infection is a systemic infection transmitted between humans by mosquitoes, mainly the species Aedes aegypti and, to lesser extent Aedes albopictus. There are total four serotypes of DENV (DENV-1, DENV-2, DENV-3 and DENV-4); each of these four serotypes of DENV is capable of causing the full spectrum of clinical manifestations that may varies from patient to patient, ranging from an asymptomatic infection to dengue fever (DF) and the most severe disease, dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS). The DENV are members of the Flavivirus genus of the Flaviviridae family; which includes in the same family are several viruses that pose a threat to public health. DENV is a positive-sense single-stranded RNA (ssRNA) virus with a genome of about 11 000 nucleotides which encodes at least 10 viral proteins including 3 structural proteins and 7 non-structural (NS) proteins. The C-terminal domain of the NS3 protein is known as NS3 helicase; NS3 helicase domain also has a nucleoside 5’-triphosphatase (NTPase) activity that responsible for ATP hydrolysis to provide the chemical energy to driven the translocation and unwinding mechanical process. The rapidly expanding footmark of dengue is a global public health challenge with an economic burden that is currently unmet by specific therapeutic agents or efficient vector control strategies. The challenges associated with the development of a successful DENV vaccine underlines the importance of antiviral development for DENV. Current study screened through 16 medicinal herbs to evaluate the possibilities of discovery of any inhibitors on the DENV-2 NS3 helicase ATPase activity. Total of 2 extracts of Polygonum chinense exhibited a certain degree of inhibition on the DENV-2 NS3 helicase ATPase activity in the plant extract screening section; these 2 extracts were further validated by conducting the dose response test. Ethyl acetate extract of Polygonum chinense exhibited inhibitory effects against the DENV-2 NS3 helicase ATPase activity in a dose-dependent manner.