Preliminary Study of Force Related Reactions Using Atomic Force Microscopy

• Main Authors: Kuo, Po-Lin, 郭柏麟
• Other Authors: Fuh, CHWAN-BOR
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/91394087725709739821

碩士 === 國立暨南國際大學 === 應用化學系 === 104 === Atomic force microscopy (AFM) is suitable to study live cells and biological systems because it has nanometer resolution, picoNewton (pN) sensitivity and without damaging the specimens. Recent developments in force-distance curve–based AFM enable one to combine sub-molecular imaging with quantitative mapping of physical, chemical and biological properties. Therefore, this thesis uses imaging of AFM to study the topography of streptavidin in buffer, and analyze force-distance curves between biotin tip of probe and streptavidin substrate by using their specific interaction to achieve the technique of molecular recognition by AFM. The results show that the percentages of force-distance curve between biotin and streptavidin are 20 to 30 % which are higher than blank (8 %) and control (12 %) respectively.