Cytotoxicity against Cancer Cells of ZnO Paper Co-deposited with Anti-Cancer Drugs at Low Temperature

• Main Authors: Li,Yueh-Lung, 李岳龍
• Other Authors: Wu,You-Lin
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/51903745119489388439

碩士 === 國立暨南國際大學 === 電機工程學系 === 104 === Zinc oxide (ZnO) nanoparticle is harmful for human health, while it has been found to exhibit cytotoxicity on several human glioma cell lines but exerting no effect on normal human astrocytes. The purpose of this research aims at investigating the feasibility of using ZnO paper prepared at low temperature with co-deposition of anti-cancer drugs for cytotoxicity effect against lung adenocarcinoma cancer cell lines A549 and H1299. Chemical reduction of zinc acetate dehydrate at 25°C and 60°C, respectively, was used to deposit ZnO nanoparticles onto the cellulose paper substrate. Some of the ZnO papers were co-deposited with anti-cancer drugs – Iressa and Staurosporine. These papers were then used as a testing tool for the cytotoxicity of ZnO nanoparticles via the MTT (3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay analysis. X-ray diffraction (XRD) was used to determine the crystallinity of ZnO nanoparticles deposited on the cellulose paper and Fourier Transform Infrared Spectroscopy (FTIR) to confirm the chemical structure of anti-cancer drugs co-deposited on the paper substrate. We used the MTT (3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay combined with enzyme-linked immunosorbent assay analysis to check the cell viability. Our experimental results confirm that ZnO nanoparticles co-deposited with anti-cancer drugs on cellulose paper can be used as a testing platform for cytotoxicity test.