| Summary: | 碩士 === 國立成功大學 === 環境醫學研究所 === 104 === Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) with nonspecific inflammatory disorder of the bowel. Although the precise etiology of UC remains unknown, the disease appears to be related with genetic and environmental factors. Tamarind-xyloglucan (TXG) is a galactoxyloglucan isolated from seed kernel of Tamarindus indica which possesses anti-oxidative property and has been used for treatment of diarrhea and gastrointestinal disorder. However, the protective effect of TXG against UC has never been studied. Therefore, the aim of this study was to investigate the protective effect and possible mechanism of TXG against UC in mice. TXG was extracted from seeds of Tamarindus indica. Dextran sulfate sodium (DSS) was administered in drinking water for 7 days to induce colitis. Simultaneously, TXG (150 or 300 mg/kg, p.o.) was given for 7 days. TXG (i) increased feed intake and colon length; (ii) decreased DAI, spleen weight, diarrhea and fecal blood; (iii) attenuated submucosal ulceration, edema, inflammatory cell infiltrations and crypt abscesses; (iv) decreased muc1, muc2 and whole mucin expression; (v) decreased TNF-α, IL-1β and IL-6, but increased IL-10; (vi) decreased NOX expression, MDA level and superoxide anion; and (vii) decreased TLR4, MyD88, p-IкB, NF-кB and COX-2 expression in DSS-treated mice. In conclusion, TXG attenuates inflammation and oxidative stress in colon mucosa by interfering TLR4/NF-κB signaling pathway, thereby it protects against ulcerative colitis in mice.
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