Comparative transcriptome analysis of Arabidopsis lyrata subsp.kamchatica from different altitude that differ in heat stress response

• Main Authors: Yung-ChuanWang, 王永全
• Other Authors: Hao-Jen Huang
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/vwz6ua

碩士 === 國立成功大學 === 生命科學系 === 104 === Because of the global average temperature has increasedin recent year, it has become an important issue in worldwide. In this study, I focus on the global warming giving an impact to alpine plants that grown in different altitudes which gives the environment diversity. The heat stress treatment of 38℃ in replacement of global warming were used to treat the Arabidopsis lyrata subsp.kamchaticafrom altitudes of 2000m and 3000m and Arabidopsisthaliana (Col-0). After treating heat stress with 38℃, Arabidopsis lyrata subsp.kamchaticafrom two altitudes and Arabidopsisthaliana (Col-0) showed different cell death ratio. NGS sequencing also showed variation in expression at transcriptome level,and found that differences of ethylene biosynthesis, calcium signaling pathway and the regulation of heat shock factor in the three plants, identifying different genes and pathway in response to heat stress. In this study also found that transcription factor and protein kinase genes that may participate in heat stress regulation in alpine plants that differ in two altitudes. Using the T-DNA mutant Arabidopsis thliana to confirm these transcription factor and protein kinase genes are participate in plant heat stress regulation. To sum up, this study presents high-alpine plants’ molecular mechanism in response to global warming. Such information is essential to identify specific genes and pathways involved in warmer climatic tolerance of high-alpine plants.