The Roles of Interleukin-8 in the Establishment of Oral Cancer Stem Cell Niche

• Main Authors: Jui-PeiWeng, 翁睿貝
• Other Authors: Kuo Yuan
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/rqf7p2

碩士 === 國立成功大學 === 生物化學暨分子生物學研究所 === 104 === Oral squamous cell carcinoma (OSCC) is the fifth most common cancer in Taiwan. Emerging evidences show that cancer stem cells (CSCs) have resistance to chemoradiation therapy and lead to tumor relapse. Moreover, there are studies suggesting that tumor microenvironment contributes to supporting CSCs and tumor vasculature. The aim of this study was to investigate how interaction between oral cancer cells and stromal cells promote cancer progression. We performed the direct and indirect contact co-culture model of SAS cells and various stromal cells. We observed that fibroblasts predominantly and strongly expressed interleukin-8 (IL-8) through contact with SAS cells. We also found that IL-8 was highly expressed in human oral cancer specimens. Immunohistochemistry analysis demonstrated that tumor tissues had higher density of microvessel and neutrophils than normal tissues. Additionally, endothelial cells up-regulated the expression of CSC-associated genes in SAS cells. Taken together, our results implied that interaction between oral cancer cells and fibroblasts may play an important role in creating the oral CSC niche via IL-8.