Detection of nucleic acid of FIV or FeLV in feline neoplastic tissues

• Main Authors: An-Hsiu Chen, 陳鞍修
• Other Authors: Shih-Chieh Chang
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/3fja4u

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 104 === Feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections induce the development of a part of lymphomas, which are one of the important causes of neoplasms in cats. Comparing to the normal population, cats infected with FIV and FeLV have higher risk to develop lymphomas. However, little is known about the impact of FIV or FeLV infection to non-lymphoid neoplasms. To address this issue, the nested polymerase chain reaction (nested-PCR) was used to detect FIV/FeLV provial sequences in feline neoplastic tissues that were collected from the Veterinary Medical Teaching Hospital, National Chung Hsing University. They included fifty non-lymphoid neoplastic specimens and five normal tissues. Primers were designed for targeting the most conserved region of pol gene of FIV, FeLV and endogenous FeLV (enFeLV). For quality control, before performing the detection for neoplastic specimens, a recombinant vector has been established to serve as the positive control in the nested-PCR for the detection of proviral sequences of FIV and FeLV. Results showed that no proviral sequences of FIV and FeLV could be detected in both non-lymphoid neoplastic specimens and normal tissues, while enFeLV could be detected in specimens. The mean positive rate of enFeLV was 50% (25/50) in all neoplastic specimens, and 51% (15/29) in mammary simple carcinomas, 58% (7/12) in feline injection site sarcomas, 33% (3/9) in other neoplasms, respectively, and 40% (2/5) in normal tissues. In conclusion, the factor of proviral sequences integration could be excluded in the oncogenesis of the neoplasms in this study. And there was no significant difference in the enFeLV positive rate among mammary carcinomas, feline injection site sarcomas, other neoplasms and normal tissues, in which no neoplastic type was considered related to the existence of this enFeLV.