Studies on Hydroxyproline Selection for Stress Tolerant Pineapples

• Main Authors: Hsin-Yu Wu, 吳馨宇
• Other Authors: 陳京城
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/76472545172082387402

碩士 === 國立中興大學 === 園藝學系所 === 104 === Using ‘Tainung No.17’ and ‘Tainung No.20’ pineapple (Ananas comosus (L.) Merrill.) as plant material, different combinations of NAA and BA were tested for callus induction, somatic embryo induction and organogenesis. The optimal treatment conditions for EMS mutagenesis and hydroxyproline selection were tested. In addition, cold tolerance and proline contents of EMS induced mutants were also examined. In callus induction and proliferation, white basal part of ‘Tainung No.17’ pineapple leaves cultured in 1ppm NAA+ 8ppm BA had a higher induction rate. The highest induction rate occurred in the treatment of 2ppm NAA + 8ppm BA for ‘Tainung No.20’ pineapple. The results of EMS mutagenesis treatment and hydroxyproline selection condition for ‘Tainung No.17’ tissue culture plantlets indicated that the survival rate was 62.5% 35days after 0.4% EMS 24 hours treatment and 47.5% after 28days of 18mM hydroxyproline treatment. For ‘Tainung No.20’ pineapple, the survival rate was 62.5% in the treatment of 0.4% EMS 24 hours, but only 27.5%sfter 28days of 18mM hydroxyproline treatment. As a result, the tolerance for ENS was similar for ‘Tainung No.17’ and ‘Tainung No.20’ pineapples, but the tolerance for hydroxyl- proline was lower for ‘Tainung No.20’ than for ‘ Tainung No.17’ pineapple. The second generation progenies (P2) of ‘Tainung No.17’ pineapple EMS mutant TN17M1-36-2 and ‘Tainung No.20’ pineapple EMS mutants TN20M1-4 and TN20M1-6 had higher proline contents than the control plantlets. The proline contents of the above mutants were significantly increased after cold treatment (5ºC, 14 days) and had higher survival rates than the control. The results showed that EMS treatment could generate stress tolerant progenies and hydroxyproline could be used for stress tolerant progeny selection.