| Summary: | 碩士 === 國立中興大學 === 動物科學系所 === 104 === Semen quality affects fertility and fecundity of ganders. Motility is one of the major characteristics of spermatozoa and is important for normal fertility. Seminal plasma provides a safe environment, nourishing and buffering properties and thus influence the structure and function of spermatozoa. Endocrine mechanisms also control the development of reproductive capacity. The purposes of this study was to investigate whether sex hormone in the serum associate with sperm motility and to explore the differentially expressed proteins in spermatozoa and seminal plasma of ganders with different sperm motility. Blood and semen samples were collected from 39 White Roman geese (age = 24 ± 2 months). Blood samples were used to determine the levels of testosterone and estrodial. Protein samples of spermatozoa and seminal plasma from semen with top 15% and bottom 15% sperm motility were subjected to isobaric tags for relative and absolute quantification (iTRAQ) analysis. The concentrations of testosterone and estradiol in serum were not differed between the two groups. Results showed that 93 out of 3,090 identified proteins had differential expression between the two groups with a minimum of 1.5-fold changes. Among these proteins, 56 proteins were upregulated and 37 proteins were downregulated in the sperm with higher motility. Gene ontology analysis revealed that most of the differentially expressed proteins were involved in biological process of cellular process (27%), regulation of biological process (24%), cellular component organization or biogenesis (11%) and metabolic process (11%). The majority of the differentially expressed proteins were located in cytoplasm (44%) and extracellular exosome (10%), and participated in molecular function of protein binding (33%), hydrolase activity (16%) and ion binding (12%). Proteins associated with energy metabolism, including L-lactate dehydrogenase, NADH dehydrogenase, adenylate kinase 8, and with spermatozoa structure, including outer dense fiber 2, tektin 2, sperm-associated antigen 6, were upregulated in sperm with higher motility. Results in the analysis of seminal plasma proteins showed that 27 out of 675 identified proteins had differential expression between the two groups with a minimum of 1.5-fold changes. Among these proteins, 14 proteins were upregulated and 13 proteins were downregulated in the seminal plasma of semen with higher sperm motility. Gene ontology analysis revealed that most of the differentially expressed proteins were involved in biological process of regulation of biological process (30%) and response to stimuli (26%). The majority of the differentially expressed proteins were located in cytoplasm (34%), and participated in molecular function of protein binding (63%) and hydrolase activity (19%). Glutathione S-transferase Mu 1 and alpha-1-antiproteinase 2 of seminal plasma were upregulated in semen with higher sperm motility. In conclusions, the results of this study suggested that sex hormone in the serum may not associate with sperm motility and expressions of proteins associated with energy metabolism, sperm structure, antioxidation and antiproteinase were higher in sperm and seminal plasma with high motility and thus regulate sperm motility of ganders.
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