| Summary: | 碩士 === 國立中興大學 === 動物科學系所 === 104 === Pork is one of the favorite ingredients. Outflow of diseased porcine tissues has been occurred, causing panic and even a health hazard to the public. Pathological tissues might be caused by many reasons, such as infection or stress. Early infections may lead to the inflammation which causes body and cell damage or external infection, stimulating and migration of the immune cells (i.e., neutrophils) and further releasing gelatinase. Gelatinase is one kind of matrix metalloproteinases. There are two types of gelatinases, in which gelatinase A (MMP-2) is synthesized by a wide variety of cells, including fibroblasts, endothelial cells, and alveolar epithelial cells, whereas gelatinase B (MMP-9) is mainly produced by the inflammatory cells. High content of procalcitonin (PCT), which is a pro-peptide of calcitonin (CT), can be an index of possible bacterial infection, bacterial infection-induced systemic inflammation, or even severe sepsis. The purpose of this study was to attempt to distinguish the normal or diseased porcine tissues through the evaluation of gelatinase activity and procalcitonin concentration. In this study, the pathological porcine tissues, including heart, kidney, liver, and muscle tissues from Boston butt and ham were collected from the abnormal hogs inspected by the veterinarian in the slaughter house, while the normal samples were collected from a supermarket and slaughter house. All samples were stored in -20℃ for 24 h and then extracted by using Triton X-100 and PBS; the former one is a nonionic surfactant which can lyses cells to extract protein or organelles and the latter one is a buffer solution that has similar osmotic and ion concentrations with the cells. In this study, SDS-page and gelatinase zymography were applied to evaluate the protein performances and the activity of gelatinases in normal and pathological tissues. Additionally, the PCT ELISA was applied to detect the PCT level (ng/mL) in the tissues. The results show that the protein content extracted by PBS was higher than that extracted by using Triton X-100. No significant difference (p<0.05) was observed on the SDS-page results between the normal and abnormal tissues. However, it was found a lower MMP-9 level in the normal liver tissues. Heart tissues had higher MMP-2 level than other tissues. The MMP-9 level in normal kidney tissues was higher whereas only MMP-2 was detected in Boston butt and ham tissues. A higher PCT content was observed in the kidney collected from the supermarket. The levels of PCT and MMP-9 in heart and liver tissues were correlated (R>0.5). In summary, MMP-9 level was noticeably higher in pathological liver tissues (p<0.05). MMP-2 was expressed in all tissues. The PCT concentrations were correlated to the MMP-9 activity in the liver and heart tissues. Differences in MMP-9 activity and procalcitonin concentration could be applied as a nonspecific indicator of early inflammation in liver tissues.
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