Evaluation of anti-bacterial and anti-biofilm formation abilities of the essential oil of Pinus morrisonicola Hayata

• Main Authors: Yen-Ju Ke, 柯彥如
• Other Authors: Hsiao-Wei, Wen
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/84085335678600544328

碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 104 === Pinus morrisonicola Hayata belongs to the pinaceae (pine family) and it is an endemic species in Taiwan. Furthermore, P. morrisonicola Hayata has been shown to have antioxidant and anti-inflammatory abilities. For purpose of evaluating whether essential oil of P. morrisonicola Hay. needle can inhibit bacterial growth and biofilm formation or not. This study applies distillation method to extract essential oil from pine needle. Antimicrobial activity was tested by MIC assay and inhibitory zone assay. Moreover, anti-biofilm formation was measured by using the dye crystal violet. The results showed that when 16200 ppm of essential oil was used, the bacteria of Streptococcus aureus , Streptococcus mutans , Streptococcus sanguinis , Streptococcus sobrinus decreased to 0%、16%、15%、7.24%, respectively. At 18000 ppm of essential oil, the growth of Sta. aureus, S.sanguinis, S. sobrinus were inhibited. Although S. mutans was not inhibited completely, the amount of bacteria was reduced to 8.25%; Moreover, at 16200 ppm of essenctial oil, biofilm formation of S. mutans, S. sanguinis, S. sobrinus was inhibited compelety. As the concentration of essential oil increased to 18000 ppm, S. mutans was inhibited completely. The result of inhibitory zone assay showed that essential oil had better ability to inhibit the growth of Sta. aureus, S. sanguinis, and S. sobrinus, and the inhibitory zones were 13.00 ± 00, 10.33 ± 0.58, and 11.67 ± 0.19 mm. However, the essential oil had smaller inhibitory zone for S. mutans which was 6.33 ± 0.58 mm. This study shows that the essential oil of P. morrisonicola Hayata can inhibit bacteria growth and can be applied to oral products in the future.