Effects of ppc and pta double knockouts on Rubisco-based Engineered Escherichia coli

• Main Authors: En-Jung Liu, 劉恩榕
• Other Authors: 李思禹
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/40783640148444861951

碩士 === 國立中興大學 === 化學工程學系所 === 104 === From the industrial revolution in the 19th century, there had so many problems on earth like global warming and climate change because of greenhouse gases. Constructed a pathway to recycle the carbon dioxide is a way to reduce the carbon dioxide emission at chemical production or fermentation industries. Incidentally, it will also increases the efficiency of processes in related bio-industries. In previous study, when form I Rubisco is heterologously expressed in E. coli, we can see the enhanced biomass. But the glucose consumption of E. coli MZL and MZLF were less than E. coli MZ, and Rubisco-based engineered pathway was not rescuing this situation. In this study, the deletion of ppc was not increased the glucose consumption, but with induced by PrkA and Rubisco were extraordinarily increase of glucose consumption. This effect would further enhanced with deletion of pta. The glucose consumption of CA1 and CA3 were 87 ± 2 and 88 ± 3 mM much than CC and CA which glucose consumption were 47 ± 2 and 49 ± 1 mM. It proved that glycolysis was enhanced by double knockouts. The Rubisco-based engineered pathway can also rescue the low glucose uptake which glucose consumption of CAB+IP can reach 60 ± 6 mM.