Monitoring truncation and ring finger region of Cbl-b in SLE patient

• Main Authors: FU,ZHAO-YANG, 傅詔揚
• Other Authors: LIU,CHAO-LIN
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/tt7fnt

碩士 === 明志科技大學 === 化學工程系生化工程碩士班 === 104 === Systemic lupus erythematosus (SLE) is an autoimmune disease. There are many factors involved in SLE but its pathogenesis is still unclear. It is reported Casitas B-lineage lymphoma B (cbl-B) plays a role in SLE. Cbl-B is an E3 ligase and a negative regulator in immunoregulator via inactivation of regulatory T cell. Among the structure of cbl-B, the ring finger motif (RF) is vital on enzyme activity. In addition, the C-termini of cbl-B from activation patients can not be identified with molecular technique. The RF of cbl-B from SLE activation patients were examined with reverse transcription PCR and sequencing. The truncation of cbl-B was also monitored by rapid amplified RNA end (RARE) technique. Among the samples, RF of cbl-b seldom mutated. However, the truncation site of cbl-B was identified. The results indicated the cbl-B from SLE activation patients was truncated. In future, the cause and function of truncated cbl-B will be explored.