Anti Dengue Virus constituents from Formosa Tithonia diversifolia

• Main Authors: Tzu-Chien Liu, 劉姿芊
• Other Authors: Yun-Sheng Lin
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/67762651350288733326

碩士 === 美和科技大學 === 生技科技系健康產業碩士班 === 104 === Dengue virus is transmitted in tropical and subtropical areas, especially in Southeast Asia and Latin America. In 2015, according to the Ministry of Health, Welfare CDC statistics, there are about 40,000 people infected with dengue virus in southern Taiwan, and more than 200 people died. Currently in strategy on the prevention and treatment of dengue fever, in addition in December 2015, Mexico and the Philippines approved for sale tetravalent dengue vaccine (Dengvaxia) , no special medical treatment. The purpose of this studies, is extracted and investigated the compound activity of Taiwan endemic plant of the stem and leaf from Tithonia diversifolia (Hemsl.) A. Gray. In this study, successfully isolate a series of natural products, including one new natural products compound A (1) and five sesquiterpene lactones, Tagitinin A (2) 、Tagitinin C (3) 、Tagitinin F (4) 、4β , 10β-dihydroxy -3-oxo-8β-isobutyroyloxyguaia-11(13) -en- 6,12-olide (5)，Tagitinin G (6) and two dicaffeoyl group structure，3,5-dicaffeoylquinic acid (7) and methyl 3,5-dicaffeoylquinate (8) from the Taiwan Tithonia diversifolia. All structures of the compounds described by a variety of NMR spectra , with physical data ( IR, mass spectrometry, ultraviolet , optical rotation ) , and compared to the data in the literature related compounds in the past to be established. Selected compounds were then investigated for their ability to inhibit dengue NS2B. Among these compounds，Tagitinin C (3) exhibited significant inhibitory effects against dengue NS2B in the infected Huh-7 cells replication in the infected cells. The future will further explore the mechanism of action and animal experiments.