| Summary: | 碩士 === 高雄醫學大學 === 醫學檢驗生物技術學系碩士班 === 104 === Analytical techniques for trace element analysis have been well developed based on the combination of various techniques and instruments. However, some limitations resulted from the selectivity, sensitivity, instrumental cost and analytical time have further improved the development of real-time methodology for trace elements. Based on the special optical characteristics, several nanomaterials have been used to separate and preconcentrate trace element species prior to detection through mass spectrometry or optical spectroscopy. The aim of the study was to develop two approaches for the determination of trace elements using two functionalized nanomaterials. Firstly we developed an eco-friendly, and highly selective method using a L-cysteine-coated magnetic gold nanoparticles (MNP@AuNPs) coupled with inductively coupled plasma mass spectrometry (ICP-MS) to separate trace Hg2+ ions from aqueous samples. The proposed method is simple and does not need desorption process when compared to the conventional solid phase extraction method. In addition, a selective colorimetric Cr(III) detection method was developed by using glutathione (GSH) functionalized gold nanoparticles (GSH@AuNPs). The presence of Cr(III) induced aggregation of GSH@AuNPs, yielding a color change from red to blue. To optimize the analytical characteristics of the two methods developed, we investigated several factors including reaction conditions of nanomaterial, adsorption pH, adsorption time/capability, coexisting ions and so on. The precision of the proposed two method is acceptable based on the results of inter-and intra-assay. Future studies are necessary to apply the proposed methods for analysis of real samples. In summary, the proposed two methods have some advantages including simple sample pretreatment and less reagent consumption, which have potentials for trace element analysis.
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