Molecular mechanism of lemongrass oil and citral induced growth inhibition and apoptosis in human malignant melanoma A375 cell lines

• Main Authors: Tzu-Yu Chou, 周子妤
• Other Authors: Kee-Lung Chang
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/15200617430014269643

碩士 === 高雄醫學大學 === 醫學系生物化學科碩士班 === 104 === Lemongrass one kind of herb, is widely used as a flavoring of food, and is used to lessen pains by it’s analgesic and anti-inflammatory properties. Previous research indicated citral is the major effective compound of lemongrass and has the potential to induce apoptosis of cells. The present study is to explore the effects of lemongrass oil and citral on human malignant melanoma cell line (A375) and explore the underline mechanism. Results showed that lemongrass oil and citral inhibited A375 cells growth in both time-dependent and dose-dependent manner. At the concentrations ranged from 30μg/ml to 50 μg/ml, either lemongrass oil or citral could stimulate production of reactive oxygen species (ROS), arrest cell cycle progress in G2/M phase by decreasing the synthesis of cyclin B1 and cdc25c, and induce apoptosis of human malignant melanoma A375 cells. Moreover, the expressions of antiapoptotic protein of Bcl-2 and Bcl-XL were down-regulated, whereas expressions of proapoptotic protein Bax, caspase-9, and caspase-3 were up-regulated.Moreover, Fas ligand and receptor expressions were also up-regulated which could activate caspase-3. Changes of those leaded to occurence of apoptosis.These results suggest that lemongrass oil and citral may have the potential in prevention against human malignant melanoma. However, further studies are needed to accumulate enough evidences to support this viewpoint.