Supercritical Fluid Extraction of Bitter Melon Seed Oil

• Main Authors: Po-Hao Lin, 林柏豪
• Other Authors: Ming-Tsai Liang
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/93453232481097199550

碩士 === 義守大學 === 化學工程學系暨生物技術與化學工程研究所 === 104 === The high level of 9cis, 11trans, 13trans-conjugated linolenic acid (9c,11t,13t-CLN) is the main oil ingredient discovered in bitter melon seeds. Other secondary oil ingredients includeunsaturated fatty acids with stearic acid and conjugated double bonds linoleic acid. Opposite to traditional solvent extraction which leaves residue in the final products, this works focuses on using supercritical fluid extraction (SFE) for the advantages of low temperature, high extraction efficiency and recycled carbon dioxide solvent in hope to achieve a greener solution.In this work, bitter melon seeds were taken as experimental material for extraction and the extraction efficiency was investigated by using different operation conditions. The parameters contain extraction temperature, pressure, flow rate of carbon dioxide, extraction time, and the holding time. The results show that much more compounds with mixed ingredients were obtained from the extraction when the temperature of the extractor is controlled at 50℃ and the pressure set as 35MPa. It is also observed that the flow rate of carbon dioxide was not a crucial factor in extracting oil from bitter melon seeds. Using ethanol as cosolvent may help the productivity but does not generate high concentration CLN bitter melon seed oil. The results prove that SFE is a novel option to extract chemical components from bitter melon seed quickly and efficiently, making SFE the foundation for the research of chemical composition in the future.