Systemic administration of Salmonella coating cytolethal distending toxin for cancer therapy

• Main Authors: Wei-Cheng Chiang, 江惟誠
• Other Authors: 王唯匡
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/31851146776504081044

碩士 === 逢甲大學 === 環境工程與科學學系 === 104 === Salmonella is a powerful antitumor agent due to its tumor-targeting potential, antitumor capability. Additionally, Salmonella can deliver a substantial amount of therapeutic molecules and continuously release them during infection. It leads to high concentrations of anti-cancer factors accumulated inside the solid tumors. These indicated that engineered bacteria are well suited for incorporating multiple targets into a single system. Cytolethal distending toxin (Cdt) B, a genotoxin produced by Campylobacter jejuni, is a DNase that causes DNA double-strand breaks (DSB) in the nucleus resulting in cell cycle arrest at the G2/M stage and apoptosis. The purpose of this study was to investigate the modulation of the tumor-targeting efficiency of Salmonella enterica serovar choleraesuis (S.C.) by coating them with CdtB, designated CdtB-S.C. In this study aimed to investigate the benefits of the use of PAH-S.C. for gene delivery. To evaluate this modulation, the cytotoxic assay CdtB-S.C. was measured both in vitro and in vivo. Treatment with CdtB- S.C. resulted in inhibiting tumor growth and prolonged the survival of tumor-bearing mice. These results suggest that tumor-targeted therapy using S.C. carrying CdtB, which exerts antitumor activities, represents a promising strategy for the treatment of tumors.