| Summary: | 碩士 === 大葉大學 === 生物產業科技學系 === 104 === Phycocyanin is a major phycobiliprotein composition produced by cyanobacteria, and it is a natural blue dye used in food and medicine industry. In the present study, we discussed the production, purification and application of phycocyanin from three isolated strains (Oscillatoria sp. Wu1, Thermosynechococcus sp. and Spirulina sp.) Light-emitting diodes (LEDs) technology has significant potential advantages over other light sources in algal culture. This study investigated LEDs as light sources for Oscillatoriales sp. from the experimental results, red LED exhibited the highest biomass (0.17 g/L) and pigments (chl (25.34 mg/L) and phy (39.28 mg/L)) under the condition of 10,000 lux. The corresponding phycocyanin yield (Yp/x) reached 0.23 g-phycocyanin/g-biomass. Red LED with light intensity of 10,000 lux had the highest pigments production and produced the highest purity ratio of phycocyanin (A620/A280=1.31). However, under green and blue LEDs light condition, the productivity of phycocyanin and biomass of the Oscillatoriales sp. are very low. On the other hand, as a control, the Oscillatoriales sp. was cultured under irradiation of white lamp (30,000 lux) for 28 days. The phycocyanin (42.44 mg/L) and chl (25.39 mg/L) contents were reached maximum at 30,000 lux. Light sources colud significantly affect the phycocyanin production. Considering both efficiency and energy cost, red LED was the optimal light source. moreover three purified phycocyanin of Oscillatoria sp. Wu1, Thermosynechococcus sp. and Spirulina sp. showed all maximum absorbance at 620 nm. The purification involves a multistep treatment of the crude extract, and tangential flow filtration and gel filtration chromatography. Three purification phycocyanin from Oscillatoria sp. Wu 1, Thermosynechococcus sp. and Spirulina sp. purity ratio (A620/A280) of 1.85, 2.57 and 3.71 were achieved, respectively. On the other hand, phycocyanin has been found to have an anti-inflammatory activity. In the study, we examined the influence of phycocyanin on lipopolysaccharide LPS-induced inflammatory reactions in mouse RAW264.7 macrophage cells. The results showed that phycocyanin had the capability to induce secretion of inflammatory cytokines, including TNF-, IL-10, and IL-6, and that these results were not due to contamination with LPS.
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