| Summary: | 博士 === 大葉大學 === 生物產業科技學系 === 104 === Ferulic acid (4-hydroxy-3-methoxy cinnamic acid, FA) belongs to the family of phenolic acids and is very abundant in grains, fruits and vegetables. It has a capacity of antioxidation, antitumor activity, potential health benefits against cardiovascular problems, inflammatory diseases and cancer. The solubility of FA in either a hydrophobic or hydrophilic solvent was low and thus limits its application. To apply natural FA in lipophilic formulation and processing by lipase synthesis of hydrophobic derivatives of FA to increase its oil-solubility, which find a variety of applications in the cosmetic and food industries. Environment-friendly preparation of hydrophobic derivatives of FA is therefore a challenge for research.
In this study have 3 parts, lipase (Novozym® 435) -catalyzed transesterification of FA and 2-Ethyl hexanol to biosynthesis 2-ethylhexyl ferulate (2-EF) was optimized.
Part I: Efficiency of biosynthesis factor for material ratio, response surface methodology (RSM) and Box-Behnken design has been used. In order to optimise the biosynthesis conditions.
Part II: Analysis of ridge max and Artificial Neural Network (ANN) has been used. Compared with RSM, intensity of the predictive value was enhanced by ANN. The ANN model for the 2-EF biosynthesis was built and the optimal synthesis condition was in a reaction time of 23 h, reaction temperature of 71 °C, enzyme amount 1,422 PLU and 99.5±1.5% conversion. Excellent biosynthesis of 2-EF under this optimal condition was reduced reaction time to hour.
Part III: From recent study report, the Maillard reaction or non-enzymatic browning/glycation is not a single reaction, but a complex series of reactions about ageing process and diabetes mellitus. Compared with Aminoguanidine hemisulfate (AG), the FA and 2-EF were inhibited glycation by SDS-PAGE. The 2-EF could effectively inhibit the glycation thanα-lipoic acid (LA). It evidenced that the FA of enzymatic esterification maintained activity and increased its application.
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